First permanent English settlement in the New World located in Virginia on the Chesapeake Bay/James River; settled by the Virginia Company of London….. ……………………First permanent English settlement in the New World located in Virginia on the Chesapeake Bay/James River; settled by the Virginia Company of London.History: Original settlers suffered from disease (especially malaria), internal strife, & starvation.Leaders:John Smith – Demanded that “He who does not work, will not eat.”John Rol